The man’s car crossed onto the wrong side of the Murray Valley Highway and hit a truck head-on about 2.45am.

He died at the scene and the truck driver, a 29-year-old Queensland man, was not injured.

The dead man has not been formally identified.

The highway is closed to all traffic between Strathmerton and Cobram and police are seeking witnesses.

Anyone with information can call Crime Stoppers on 1800 333 000 or visit crimestoppersvic.com.au