Victoria Police are on scene at a fatality on Chiltern Valley Road, Chiltern Valley.
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Media officer Belinda Batty said a single car was involved in the fatal crash.
She said the car left Chiltern Valley Road and struck a tree just after 3pm and caught fire.
Police said the man driving the car was pronounced dead at the scene but has yet to be identified.
Anyone who witnessed the collision is asked to contact Crime Stoppers on 1800 333 000 or submit an anonymous report at www.crimestoppersvic.com.au