Victoria has recorded another 484 cases of coronavirus in the last 24 hours.
It marks the highest number of cases recorded in a single day in Victoria.
The previous record was 428 which was reported last Friday.
Victorian Premier Daniel Andrews said 97 of the 484 cases were connected to known outbreaks.
He said two men in their 90s had died overnight bringing the total of deaths to 44 Victorians.
It comes as new border restrictions between NSW and Victoria, despite the region only having three cases of COVID-19.
There have been no new cases of COVID-19 in the Border or North East and Murrumbidgee Local Health District have conducted 30,000 tests in the region.
A new active case of coronavirus has been recorded in Mansfield Shire, but the Department of Health and Human Services and the Mansfield Hospital have been unable to clarify if the result was someone currently in the area or elsewhere with a home address in Mansfield.
Doctors have urged Australian governments to adopt a consistent approach to face masks and to strengthen border controls to stop coronavirus.
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Australian Medical Association president Tony Bartone said every person in areas of community transmission should use masks.
"The Victorian and NSW governments are to be commended for encouraging mask use, but a consistent national cabinet-backed approach is now needed should outbreaks occur elsewhere," he said.
NSW Premier Gladys Berejiklian has stopped short of making face coverings mandatory, as they will be in Melbourne and Mitchell Shire from Thursday.
Queensland is considering expanding its blacklist of NSW virus hotspots.
Victoria recorded another 374 cases on Tuesday and three more people died.
NSW confirmed 13 new cases, just one of whom was in hotel quarantine.
- with AAP