Wangaratta's lead for the league title has been reduced to two points after it fell 3-1 to Melrose on Sunday.
After drawing with Cobram last weekend, the Red Devils suffered their first loss of the season against the eighth-placed Melrose.
Reigning league and cup champions Myrtleford are ready to pounce after beating Albury Hotspurs 3-1 to jump within two of the lead.
Albury United missed a golden opportunity to move one off the pace after falling 2-0 to Albury City.
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Twin City hammered the in-form Cobram 6-0 at home thanks to a hat trick from the talented Liam Walsh.
Boomers defeated St Pats 3-0, while Wodonga Diamonds took down Wodonga Heart 7-0.
The senior men's ladder after round 18 is: Wangaratta 45, Myrtleford 43, Albury United 41, Albury City 40, Boomers 37, Cobram 31, Twin City 24, Melrose 20, Albury Hotspurs 14, Wodonga Diamonds 10, St Pats 6, Wodonga Heart 3.
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