Four cases of whooping cough have been confirmed in Beechworth in the past week.
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Three of those cases were students at Beechworth Montessori School with parents being notified on Monday night two students had contracted the respiratory infection after one last week.
Principal Susi Allen said the independent school, which educated around 80 students, notified the Department of Health and Human Services of the confirmed cases of whooping cough, or pertussis.
"This is mandated by the regulations under the Public Health and Wellbeing Regulations 2009, which is school policy," she said.
"The school also mandates the Victorian law, under the No Jab, No Play legislation, when enrolling a child.
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"Beechworth Montessori has strong policies and implementation as acknowledged in the recent assessment and rating report of the National Quality Standard by the Australian Children's Education and Care Quality Authority in which we received an overall rating of exceeding national quality standard."
All cases are being described as mild with majority of cases having been vaccinated against the infection.
Beechworth GP Christie Rodda said despite seeing cases of pertussis in vaccinated people vaccination is still the best.
"Vaccination is still the community's best defence against the spread of pertussis to vulnerable groups," she said.
Dr Rodda said all cases will be managed in accordance with Department of Health guidelines which includes exclusion periods.