Some fitness centres that operate 24 hours north of the border have been forced to scale back following NSW Premier Gladys Berejiklian's announcement that all gyms must have a hygiene marshall on site at all times.

Anytime Fitness North Albury has reduced to 15 hours, while Plus Fitness in Thurgoona has cut back to 5am to 9pm on weekdays and less on weekends to comply with the new safety measures.

But both gyms admit they're still in a better position than those south of the border, who now face their second closure this year due to a return to stage three lockdown in regional Victoria.

"If we want to stay open this is what we need to do," Anytime Fitness manager Sam Hollis said.

"We're now operating between 6am and 9pm during the week, which as a result has created a bottleneck because you're trying to fit more people in a shorter period of time.

"The wages have gone up because you're paying someone to be here for those 15 hours a day, where as before it might have been 10.

"Everyone's been really good with hygiene, wiping down equipment, bringing towels and social distancing.

"We've embraced the new gym life. It's not the same."

Plus Fitness owner Simon Hodgson agreed it wasn't viable to continue to operate for 24 hours, but said the changes hadn't impacted memberships.

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"People appreciate that we can still be open," Hodgson said.

Owner of PT Fitness in Wodonga Nic Conway said it was disappointing to have to close the door on members again.

"For us, and a lot of gyms, we have a lot of people involved in local football and netball," Conway said.

"The gym had been that void for missing their team sport.

"It's unusual that a gym is really busy in the middle of winter."

Conway said trainers will now look to an online approach for clients, something that worked well for them last lockdown.

"People complimented our trainers in helping them get through what is a tough time," he said.

The Klinik owner and exercise physiologist Erin Glass said she would also be offering online classes while the Wodonga pilates and wellness centre is closed.

"Our motto is movement is medicine, so if I can help in some way with a 45 minute workout online, I'll do that," Glass said.

"It's been harder this time personally because we've had to say goodbye to clients over the last few days.

"We've got such a beautiful community, so saying goodbye to them has been really hard."

Glass said she saw an increase in members after the first shut down.

"I think people's priorities have changed and they're more inclined to look after their health, both physically and mentally," she said.

With the lockdown expected for six weeks at this stage, Glass hopes to open in time to celebrate the Klinik's first birthday.