Passenger services on the Albury to Melbourne line have been cancelled and replaced with coaches.
V/Line confirmed late Thursday a driver took a test Wednesday and had been isolating, but had been working between Saturday and Tuesday with no symptoms.
An operations depot at Southern Cross Station he attended on Monday and Tuesday was evacuated on Thursday and has been deep-cleaned, as well as Traralgon station, where the driver spent time in a recreation room on Monday.
The Southern Cross depot, as well as all trains, are regularly cleaned and sanitised, according to V/Line.
All staff who are close contacts have been contacted and will be required to isolate until they receive a negative test result.
IN OTHER NEWS:
"We are closely monitoring staff availability and we are working hard to minimise any impact to services. At this stage, there has not been any service changes due to staff undertaking precautionary isolation and COVID-19 testing," a V/Line spokesperson said in a statement.
"The health and welfare of our staff and passengers is our top priority, and we are working closely with the Department of Transport and Department of Health to ensure the appropriate actions are taken in response."
View current exposure sites at coronavirus.vic.gov.au.
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